IL1RL2 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-1 Rrp-2, hFc Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 20.9 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-1 Rrp2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human IL1RL2 (Q9HB29-1, Asp20-Arg335) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Asp20-Arg335
Host
Human
Theoretical MW (kDa)
62.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 20.9 ng/mL, messured by ELISA at 1 ug/mL. The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-1 Rrp2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-1 Rrp-2, hFc Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 20.9 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 uM as determined in SPR assay (Biacore T200). -
Gene Info — IL1RL2
Entrez GeneID
8808Protein Accession#
Q9HB29-1Gene Name
IL1RL2
Gene Alias
IL1R-rp2, IL1RRP2
Gene Description
interleukin 1 receptor-like 2
Omim ID
604512Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq
Other Designations
interleukin-1 receptor-related protein 2
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Interactome
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Disease
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