SERPINF2 (Human) Recombinant Protein
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Specification
Product Description
Human SERPINF2 (P08697-1, Met28-Lys491) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Met28-Lys491
Host
Human
Theoretical MW (kDa)
52.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.27 ug/mL, messured by ELISA at 5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human SERPINF2 is greater than 90% as determined by SEC-HPLC.
Tris-Bis PAGE
Human SERPINF2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human SERPINF2, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 0.27 ug/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — SERPINF2
Entrez GeneID
5345Protein Accession#
P08697-1Gene Name
SERPINF2
Gene Alias
A2AP, AAP, ALPHA-2-PI, API, PLI
Gene Description
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Omim ID
262850Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
alpha-2-antiplasmin|alpha-2-plasmin inhibitor|serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
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Interactome
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