TNFRSF8 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD30 Ligand, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 8.9 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human CD30 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human TNFRSF8 (P28908-1, Phe19-Lys379) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Phe19-Lys379
Host
Human
Theoretical MW (kDa)
65.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.9 ng/mL, messured by ELISA at 5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD30 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD30 Ligand, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 8.9 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — TNFRSF8
Entrez GeneID
943Protein Accession#
P28908-1Gene Name
TNFRSF8
Gene Alias
CD30, D1S166E, KI-1
Gene Description
tumor necrosis factor receptor superfamily, member 8
Omim ID
153243Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
CD30 antigen|CD30L receptor|Ki-1 antigen|OTTHUMP00000001783|cytokine receptor CD30|lymphocyte activation antigen CD30
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Interactome
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Pathway
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Disease
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