KLK5 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Kallikrein 5, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 9.4 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Kallikrein 5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human KLK5 (Q9Y337, Val23-Ser293) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Val23-Ser293
Host
Human
Theoretical MW (kDa)
30.7
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 16.8 ng/mL, messured by ELISA at 1 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Kallikrein 5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Kallikrein 5, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 9.4 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — KLK5
Entrez GeneID
25818Protein Accession#
Q9Y337Gene Name
KLK5
Gene Alias
KLK-L2, KLKL2, SCTE
Gene Description
kallikrein-related peptidase 5
Omim ID
605643Gene Ontology
HyperlinkGene Summary
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
kallikrein 5|kallikrein-like protein 2|stratum corneum tryptic enzyme
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Interactome
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Disease
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