CLEC1A (Human) Recombinant Protein
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Specification
Product Description
Human CLEC1A (Q8NC01, Gln74-Asp280) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.
Sequence
Gln74-Asp280
Host
Human
Theoretical MW (kDa)
51.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.8 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
Tris-Bis PAGE
Tris-Bis PAGE
Human CLEC-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CLEC-1, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 8.8 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CLEC1A
Entrez GeneID
51267Protein Accession#
Q8NC01Gene Name
CLEC1A
Gene Alias
CLEC1, MGC34328
Gene Description
C-type lectin domain family 1, member A
Omim ID
606782Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded protein may play a role in regulating dendritic cell function. Alternative splice variants have been described but their biological nature has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq
Other Designations
C-type lectin-like receptor-1
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