CLEC4A (Human) Recombinant Protein
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Specification
Product Description
Human CLEC4A (Q9UMR7-1, Gln70-Leu237) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
Gln70-Leu237
Host
Human
Theoretical MW (kDa)
46.7
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 16.0 ng/mL, messured by ELISA at 0.2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CLEC4A is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CLEC4A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CLEC4A, hFc Tag at 0.2 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC4A Antibody, hFc Tag with the EC50 of 16.0 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CLEC4A
Entrez GeneID
50856Protein Accession#
Q9UMR7-1Gene Name
CLEC4A
Gene Alias
CLECSF6, DCIR, DDB27, HDCGC13P, LLIR
Gene Description
C-type lectin domain family 4, member A
Omim ID
605306Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in inflammatory and immune response. Multiple transcript variants encoding distinct isoforms have been identified for this gene. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq
Other Designations
C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 6|dendritic cell immunoreceptor|lectin-like immunoreceptor
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