ALPP (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human Alkaline Phosphatase (Placental type) , His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 11.1 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Alkaline Phosphatase (Placental type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human ALPP (P05187, Ile23-Asp506) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Ile23-Asp506
Host
Human
Theoretical MW (kDa)
53.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 11.1 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Alkaline Phosphatase (Placental type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Alkaline Phosphatase (Placental type) , His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 11.1 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — ALPP
Entrez GeneID
250Protein Accession#
P05187Gene Name
ALPP
Gene Alias
ALP, FLJ61142, PALP, PLAP
Gene Description
alkaline phosphatase, placental (Regan isozyme)
Omim ID
171800Gene Ontology
HyperlinkGene Summary
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq
Other Designations
alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase
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Interactome
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Pathway
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Disease
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