FLT4 (Human) Recombinant Protein
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Specification
Product Description
Human FLT4 (P35916-1, Tyr25-Ile776) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Tyr25-Ile776
Host
Human
Theoretical MW (kDa)
111.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human VEGF R3 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human VEGF R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human VEGF-C, His Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 17.9 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — FLT4
Entrez GeneID
2324Protein Accession#
P35916-1Gene Name
FLT4
Gene Alias
FLT41, LMPH1A, PCL, VEGFR3
Gene Description
fms-related tyrosine kinase 4
Gene Ontology
HyperlinkGene Summary
This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq
Other Designations
soluble VEGFR3 variant 1|soluble VEGFR3 variant 2|soluble VEGFR3 variant 3|vascular endothelial growth factor receptor 3
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