FLT4 (Human) Recombinant Protein
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Specification
Product Description
Human PDK4 (NP_002603.1, 1 a.a. - 411 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
83
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
59 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : CSKtide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — FLT4
Entrez GeneID
2324Protein Accession#
NP_002011.1Gene Name
FLT4
Gene Alias
FLT41, LMPH1A, PCL, VEGFR3
Gene Description
fms-related tyrosine kinase 4
Gene Ontology
HyperlinkGene Summary
This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq
Other Designations
soluble VEGFR3 variant 1|soluble VEGFR3 variant 2|soluble VEGFR3 variant 3|vascular endothelial growth factor receptor 3
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