HLA-A/B2M/AFP Tetramer (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HLA-A*02:01 & B2M & AFP (FMNKFIYEI) Tetramer, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-HLA-A*02:01 & B2M & AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 9 ng/mL determined by ELISA (QC Test).
SEC-HPLC
The purity of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.
-
Specification
Product Description
Human HLA-A | B2M | AFP Tetramer (A0A140T913|P61769|P02771, Gly25-Thr305 | Ile21-Met119 | FMNKFIYEI) partial recombinant protein with His-Avi tag at C-terminus expressed in HEK293 cells.
Sequence
Gly25-Thr305 | Ile21-Met119 | FMNKFIYEI
Host
Human
Theoretical MW (kDa)
258
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS pH 7.4 (8% trehalose)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HLA-A*02:01 & B2M & AFP (FMNKFIYEI) Tetramer, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-HLA-A*02:01 & B2M & AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 9 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
-
Gene Info — AFP
Entrez GeneID
174Protein Accession#
A0A140T913(HLA-A*02:01) | P61769(B2M) | FMNKFIYEIGene Name
AFP
Gene Alias
FETA, HPAFP
Gene Description
alpha-fetoprotein
Omim ID
104150Gene Ontology
HyperlinkGene Summary
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
Other Designations
OTTHUMP00000160480|alpha-1-fetoprotein|alpha-fetoglobulin
-
Gene Info — B2M
Entrez GeneID
567Protein Accession#
A0A140T913(HLA-A*02:01) | P61769(B2M) | FMNKFIYEIGene Name
B2M
Gene Alias
-
Gene Description
beta-2-microglobulin
Gene Ontology
HyperlinkGene Summary
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia
Other Designations
beta chain of MHC class I molecules|beta-2-microglobin
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com