GAS6 (Human) Recombinant Protein
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Specification
Product Description
Human GAS6 (Q14393-2, Ala49-Ala678) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Ala49-Ala678
Host
Human
Theoretical MW (kDa)
70.59999999999989
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 12.3 ng/mL, messured by ELISA at 5 ug/mL. The affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human GAS6 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human GAS6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution of PBS, pH 7.4 (8% trehalose).
Trehalose is added as protectant before lyophilization.Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human GAS6, His Tag at 5 ug/mL (100 uL/mL/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 uM as determined in SPR assay (Biacore T200). -
Gene Info — GAS6
Entrez GeneID
2621Protein Accession#
Q14393-2Gene Name
GAS6
Gene Alias
AXLLG, AXSF, DKFZp666G247, FLJ34709
Gene Description
growth arrest-specific 6
Omim ID
600441Gene Ontology
HyperlinkGene Summary
This gene product is a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
AXL receptor tyrosine kinase ligand|AXL stimulatory factor|OTTHUMP00000018777
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