GAS6 (Human) Recombinant Protein

Catalog # P9752

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Size:100 ug
Price: USD $ 531.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

Immobilized Human GAS6, His Tag at 5 ug/mL (100 uL/mL/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3 ng/mL determined by ELISA (QC Test).

Surface Plasmon Resonance
Application

Surface Plasmon Resonance

Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).

SEC-HPLC
QC Test

SEC-HPLC

The purity of Human GAS6 is greater than 95% as determined by SEC-HPLC.

Tris-Bis PAGE
QC Test

Tris-Bis PAGE

Human GAS6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

  • Specification

    Product Description

    Human GAS6 (Q14393-2, Ala49-Ala678) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

    Sequence

    Ala49-Ala678

    Host

    Human

    Theoretical MW (kDa)

    70.59999999999989

    Form

    Lyophilized

    Preparation Method

    Mammalian cell (HEK293) expression system

    Purity

    > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

    Endotoxin Level

    < 1 EU per 1 ug of protein (determined by LAL method)

    Activity

    The EC50 was 12.3 ng/mL, messured by ELISA at 5 ug/mL. The affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).

    Quality Control Testing

    SEC-HPLC and Tris-Bis PAGE

    SEC-HPLC

    The purity of Human GAS6 is greater than 95% as determined by SEC-HPLC.

    Tris-Bis PAGE

    Human GAS6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

    Recommend Usage

    Biological Activity
    ELISA
    SPR
    SDS-PAGE
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from 0.22 um filtered solution of PBS, pH 7.4 (8% trehalose).
    Trehalose is added as protectant before lyophilization.

    Storage Instruction

    Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Result of bioactivity analysis

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Immobilized Human GAS6, His Tag at 5 ug/mL (100 uL/mL/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3 ng/mL determined by ELISA (QC Test).

    Functional Study

    SDS-PAGE

    Surface Plasmon Resonance

    Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).
  • Gene Info — GAS6

    Entrez GeneID

    2621

    Protein Accession#

    Q14393-2

    Gene Name

    GAS6

    Gene Alias

    AXLLG, AXSF, DKFZp666G247, FLJ34709

    Gene Description

    growth arrest-specific 6

    Omim ID

    600441

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene product is a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

    Other Designations

    AXL receptor tyrosine kinase ligand|AXL stimulatory factor|OTTHUMP00000018777

  • Interactome
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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