TNFRSF11A (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human RANKL, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 8.5 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human TNFRSF11A is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human TNFRSF11A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human TNFRSF11A (Q9Y6Q6-1, 30 a.a. - 212 a.a.) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
IAPPCTSEKHYEHLGRCCNKCEPGKYMSSKCTTTSDSVCLPCGPDEYLDSWNEEDKCLLHKVCDTGKALVAVVAGNSTTPRRCACTAGYHWSQDCECCRRNTECAPGLGAQHPLQLNKDTVCKPCLAGYFSDAFSSTDKCRPWTNCTFLGKRVEHHGTEKSDAVCSSSLPARKPPNEPHVYLP
Host
Human
Theoretical MW (kDa)
46.85
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.5 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human TNFRSF11A is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human TNFRSF11A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human RANKL, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 8.5 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — TNFRSF11A
Entrez GeneID
8792Protein Accession#
Q9Y6Q6-1Gene Name
TNFRSF11A
Gene Alias
CD265, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, RANK, TRANCER
Gene Description
tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Studies of the mouse counterpart suggest that this receptor directly mediates the osteoprotegerin ligand (OPGL)-induced osteoclastogenesis in osteoclast precursor cells. [provided by RefSeq
Other Designations
loss of heterozygosity, 18, chromosomal region 1|osteoclast differentiation factor receptor|receptor activator of nuclear factor-kappa B|tumor necrosis factor receptor superfamily, member 11a|tumor necrosis factor receptor superfamily, member 11a, activat
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Interactome
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Pathway
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Disease
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