TGFB3 (Human) Recombinant Protein
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Specification
Product Description
Human TGFB3 (P10600-1, 24 a.a. - 412 a.a.) partial recombinant protein with His tag at N-terminus expressed in HEK293 cells.
Sequence
LSTCTTLDFGHIKKKRVEAIRGQILSKLRLTSPPEPTVMTHVPYQVLALYNSTRELLEEMHGEREEGCTQENTESEYYAKEIHKFDMIQGLAEHNELAVCPKGITSKVFRFNVSSVEKNRTNLFRAEFRVLRVPNPSSKRNEQRIELFQILRPDEHIAKQRYIGGKNLPTRGTAEWLSFDVTDTVREWLLRRESNLGLEISIHCPCHTFQPNGDILENIHEVMEIKFKGVDNEDDHGRGDLGRLKKQKDHHNPHLILMMIPPHRLDNPGQGGQRKKRALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS
Host
Human
Theoretical MW (kDa)
45.9
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 12.1 ng/mL, messured by ELISA at 1 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Latent TGF beta 3 is greater than 95% as determined by SEC-HPLC.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Latent TGF beta 3, His Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 12.1 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — TGFB3
Entrez GeneID
7043Protein Accession#
P10600-1Gene Name
TGFB3
Gene Alias
ARVD, FLJ16571, TGF-beta3
Gene Description
transforming growth factor, beta 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq
Other Designations
transforming growth factor-beta 3
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Interactome
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Pathway
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Disease
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