TGFB3 (Human) Recombinant Protein
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Specification
Product Description
Human TGFB3 recombinant protein with His tag in N-terminus expressed in Nicotiana benthamiana.
Sequence
HHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS
Host
Nicotiana benthamiana
Theoretical MW (kDa)
27.2
Form
Lyophilized
Preparation Method
Nicotiana benthamiana expression system
Purification
chromatographic
Purity
> 97% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 40 ng/mL, measured in culture by the ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation, corresponding to a specific activity of 2.5 x 104 IU/mg.
Storage Buffer
Protein (1 mg/mL) was lyophilized from a solution containing 50 mM Tris-HCl, pH 7.4. Reconstitute the lyophilized powder ine 5 mM HCl, 50 ug/mL BSA to 0.05mg/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — TGFB3
Entrez GeneID
7043Protein Accession#
P10600Gene Name
TGFB3
Gene Alias
ARVD, FLJ16571, TGF-beta3
Gene Description
transforming growth factor, beta 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq
Other Designations
transforming growth factor-beta 3
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