RAET1L (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human NKG2D, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 5.7 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human ULBP-6 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human ULBP-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human RAET1L (Q5VY80, 26 a.a. - 218 a.a.) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
RRDDPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGNKTVTPVSPLGKKLNVTMAWKAQNPVLREVVDILTEQLLDIQLENYTPKEPLTLQARMSCEQKAEGHSSGSWQFSIDGQTFLLFDSEKRMWTTVHPGARKMKEKWENDKDVAMSFHYISMGDCIGWLEDFLMGMDSTLEPSAGAPLAMSSG
Host
Human
Theoretical MW (kDa)
48.61
Form
Liquid
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 5.7 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human ULBP-6 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human ULBP-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS pH 7.4
Storage Instruction
Store at -80°C for 12 Month.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human NKG2D, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 5.7 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — RAET1L
Entrez GeneID
154064Protein Accession#
Q5VY80Gene Name
RAET1L
Gene Alias
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Gene Description
retinoic acid early transcript 1L
Omim ID
611047Gene Ontology
HyperlinkGene Summary
RAET1L belongs to the RAET1 family of major histocompatibility complex (MHC) class I-related genes, which are located within a 180-kb cluster on chromosome 6q24.2-q25.3. The REAT1 genes encode glycoproteins that contain extracellular alpha-1 and alpha-2 domains, but they lack the membrane proximal Ig-like alpha-3 domain. Most RAET1 glycoproteins are anchored to the membrane via glycosylphosphatidylinositol (GPI) linkage (Radosavljevic et al., 2002 [PubMed 11827464]).[supplied by OMIM
Other Designations
OTTHUMP00000017409
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Interactome
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Pathway
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Disease
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