HBEGF (Human) Recombinant Protein
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Specification
Product Description
Human HBEGF recombinant protein expressed in Escherichia coli.
Sequence
MDLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSL
Host
Escherichia coli
Theoretical MW (kDa)
9.9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 95% as determined by SDS-PAGE.
Activity
ED50 is 0.13-0.2 ng/mL, determined by the ability to induce proliferation of 3T3 cells, corresponding to a specific activity of 7.7 x 106 units/mg.
Storage Buffer
Protein (1 mg/mL) was lyophilized from a solution containing 10mM sodium phosphate, pH 7.5. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — HBEGF
Entrez GeneID
1839Protein Accession#
Q99075Gene Name
HBEGF
Gene Alias
DTR, DTS, DTSF, HEGFL
Gene Description
heparin-binding EGF-like growth factor
Omim ID
126150Gene Ontology
HyperlinkOther Designations
diphtheria toxin receptor (heparin-binding EGF-like growth factor)|diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)|heparin-binding epidermal growth factor
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