HBEGF (Human) Recombinant Protein
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Specification
Product Description
Human HBEGF (Q99075, 63 a.a. - 148 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
DLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAP SCICHPGYHGERCHGLSL
Host
Escherichia coli
Theoretical MW (kDa)
9.69999999999999
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 97% as analyzed by SDS-PAGE.
> 97% as analyzed by HPLC.Endotoxin Level
< 1 EU/ug of protein by LAL method
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — HBEGF
Entrez GeneID
1839Protein Accession#
Q99075Gene Name
HBEGF
Gene Alias
DTR, DTS, DTSF, HEGFL
Gene Description
heparin-binding EGF-like growth factor
Omim ID
126150Gene Ontology
HyperlinkOther Designations
diphtheria toxin receptor (heparin-binding EGF-like growth factor)|diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)|heparin-binding epidermal growth factor
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