Fgf1 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Fgf1 recombinant protein expressed in Escherichia coli.
Sequence
MFNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYLAMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Host
Escherichia coli
Theoretical MW (kDa)
15.9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Chromatography
Purity
> 95% as determined by SDS-PAGE.
Activity
ED50 <0.2 ng/mL, measured by dose-dependent proliferation of mouse BALB/c 3T3 cells, corresponding to a specific activity of 5 x 106 IU/mg.
Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 7.5. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Stored at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C for long term storage.
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Fgf1
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