Fgf1 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Fgf1 (P61148, 16 a.a. - 155 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
FNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYLAMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Host
Escherichia coli
Theoretical MW (kDa)
16
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Activity
The ED50 was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <= 10 ng/mL, corresponding to a specific activity of >= 1 x 105 units/mg.
Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at -20°C on dry atmosphere for 2 years. After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Fgf1
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