Products

Product Description

Human TNFSF14 recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins

Sequence

MRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV with polyhistidine tag at the C-terminus.

Host

Escherichia coli

Form

Lyophilized

Preparation Method

Escherichia coli expression system

Purification

Ni-NTA chromatography

Purity

> 98% as determined by SDS-PAGE.

Endotoxin Level

< 0.01 EU/ ug of protein by the LAL method.

Activity

ED₅₀ < 23 ng/mL, Measured by the induction of cytotoxicity in HT-29 cells in the presence of IFN-gamma.ED₅₀ < 3 ng/mL, Measured by the induction of proliferation in HUVEC cells.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue.

SDS-PAGE analysis of TNFSF14 (Human) Recombinant Protein.

Recommend Usage

Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from a solution containing 0.1% sarkosyl in 1X PBS, pH 8.0. Reconstitute the lyophilized powder in ddH₂O to 100 ug/mL.

Storage Instruction

Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.

Note

Result of activity analysis