Proinsulin/INS (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
Proinsulin/INS (Human) Recombinant Protein in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4ug/mL.
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Specification
Product Description
Human Proinsulin/INS (NP_000198, 25 a.a. - 110 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Host
Human
Theoretical MW (kDa)
11.8
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
> 1 EU per 1ug of protein (determined by LAL method)
Activity
Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4 ug/ml.
Quality Control Testing
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol)
Storage Instruction
Stored at 2°C to 8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — INS
Entrez GeneID
3630Protein Accession#
NP_000198Gene Name
INS
Gene Alias
ILPR, IRDN
Gene Description
insulin
Gene Ontology
HyperlinkGene Summary
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq
Other Designations
OTTHUMP00000011161|OTTHUMP00000011162|OTTHUMP00000196038|proinsulin
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