Products

Product Description

Rat Il1b recombinant protein expressed in Escherichia coli.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins

Sequence

MVPIRQLHCRLRDEQQKCLVLSDPCELKALHLNGQNISQQVVFSMSFVQGETSNDKIPVALGLKGLNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKTKVEFESAQFPNWYISTSQAEHRPVFLGNSNGRDIVDFTMEPVSS

Host

Escherichia coli

Theoretical MW (kDa)

17.3

Form

Lyophilized

Preparation Method

Escherichia coli expression system

Endotoxin Level

< 0.1 EU/ug

Activity

Recombinant rat Il1b is fully biologically active when compared to a standard. The ED₅₀, as determined by the dose-dependent proliferation of mouse D10S cells, is 40.2 pg/mL.

Quality Control Testing

1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue

Lane 1: non-reducing conditions
Lane 2: reducing conditions

Storage Buffer

Lyophilized from 10 mM NaP, pH 7.5

Storage Instruction

Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.