His tag monoclonal antibody, clone HIS.H8
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More Files
- More Functions
Western Blot (Transfected lysate)
Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2). Blotting with His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752).
Immunofluorescence
Immunofluorescence staining of HEK293 cells.
A: untransfected control.
B: His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752) (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of His tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Host
Mouse
Specificity
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG2b
Recommend Usage
Western Blot (1:1000-1:3000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2). Blotting with His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752).Immunohistochemistry
Immunofluorescence
Immunofluorescence staining of HEK293 cells.
A: untransfected control.
B: His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752) (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot
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