FLT3 monoclonal antibody, clone BV10A4
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More Files
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Flow Cytometry
Flow cytometric analysis of FLT3 in K-562 cells with 5 ug/mL of FLT3 monoclonal antibody, clone BV10A4 (Cat # MAB8212).
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Specification
Product Description
Mouse monoclonal antibody raised against native FLT3.
Immunogen
Native purified FLT3 from BV-173 leukemic cell line.
Host
Mouse
Theoretical MW (kDa)
130-160
Reactivity
Human
Specificity
This antibody reacts with FLT3, a 130-160 KDa type III receptor tyrosine kinase that is involved in early steps ofhematopoiesis.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Flow Cytometry (10 ug/mL per 106 cells/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of FLT3 in K-562 cells with 5 ug/mL of FLT3 monoclonal antibody, clone BV10A4 (Cat # MAB8212). -
Gene Info — FLT3
Entrez GeneID
2322Gene Name
FLT3
Gene Alias
CD135, FLK2, STK1
Gene Description
fms-related tyrosine kinase 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq
Other Designations
CD135 antigen|FL cytokine receptor|FLT3 receptor tyrosine kinase|OTTHUMP00000042340|fetal liver kinase 2|growth factor receptor tyrosine kinase type III|stem cell tyrosine kinase 1|tyrosine-protein kinase receptor FLT3
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Interactome
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Pathway
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Disease
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Publication Reference
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Detailed analysis of FLT3 expression levels in acute myeloid leukemia.
Kuchenbauer F, Kern W, Schoch C, Kohlmann A, Hiddemann W, Haferlach T, Schnittger S.
Haematologica 2005 Dec; 90(12):1617.
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Inhibition of FLT3 signaling targets DCs to ameliorate autoimmune disease.
Whartenby KA, Calabresi PA, McCadden E, Nguyen B, Kardian D, Wang T, Mosse C, Pardoll DM, Small D.
PNAS 2005 Nov; 102(46):16741.
Application：WB-Ce, Mouse, Bone marrow dendritic cells.
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Increased recruitment of hematopoietic progenitor cells underlies the ex vivo expansion potential of FLT3 ligand.
Haylock DN, Horsfall MJ, Dowse TL, Ramshaw HS, Niutta S, Protopsaltis S, Peng L, Burrell C, Rappold I, Buhring HJ, Simmons PJ.
Blood 1997 Sep; 90(6):2260.
Application：Flow Cyt, Human, Human bone marrow cells.
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Detailed analysis of FLT3 expression levels in acute myeloid leukemia.
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