GFP monoclonal antibody, clone 1A5
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More Files
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Immunofluorescence
GFP-expressed in COS-1 cells (A) and immunofluorescent staing of the same sample with GFP monoclonal antibody, clone 1A5 (Cat # MAB6746) (B).
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Specification
Product Description
Rat monoclonal antibody raised against recombinant GFP.
Immunogen
Recombinant protein corresponding to Aequorea victoria GFP.
Host
Rat
Reactivity
Jellyfishes
Specificity
This antibody is specific to GFP and GFP-fused proteins.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
ChIP
Western Blot
Immunocytochemistry
Immunofluorescence
GFP-expressed in COS-1 cells (A) and immunofluorescent staing of the same sample with GFP monoclonal antibody, clone 1A5 (Cat # MAB6746) (B).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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The green fluorescent protein.
Tsien RY.
Annual Review of Biochemistry 1998 Jan; 67:509.
Application：WB-Tr, Human, Mammalian cells.
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Green fluorescent protein as a marker for gene expression.
Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC.
Science 1994 Feb; 263(5148):802.
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Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea.
SHIMOMURA O, JOHNSON FH, SAIGA Y.
Journal of Cellular and Comparative Physiology 1962 Jun; 59:223.
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The green fluorescent protein.
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