XRCC5 monoclonal antibody, clone MEM-54
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More Files
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Immunofluorescence
Immunofluorescence staining of HeLa (human cervix carcinoma cell line) using XRCC5 monoclonal antibody, clone MEM-54 (Cat # MAB6468) (detection by Goat anti-mouse IgG2a Alexa Fluor® 488 ; green).
Actin (red) was stained with Phalloidin-TRITC, nuclei with DAPI (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against native XRCC5.
Immunogen
Native purified XRCC5 from human whole T-lymphocytes.
Host
Mouse
Reactivity
Human
Specificity
This antibody reacts with Ku80, a 80 KDa subunit of Ku autoantigen (heterodimer of72 and 87 KDa polypeptides). Ku autoantigen is involved in DNA repair and in V(D) Jrecombination through its ability to bind DNA double-strand breaks.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Immunocytochemistry (2 ug/mL)
Immunofluorescence
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of HeLa (human cervix carcinoma cell line) using XRCC5 monoclonal antibody, clone MEM-54 (Cat # MAB6468) (detection by Goat anti-mouse IgG2a Alexa Fluor® 488 ; green).
Actin (red) was stained with Phalloidin-TRITC, nuclei with DAPI (blue).Immunoprecipitation
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Gene Info — XRCC5
Entrez GeneID
7520Gene Name
XRCC5
Gene Alias
FLJ39089, KARP-1, KARP1, KU80, KUB2, Ku86, NFIV
Gene Description
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Omim ID
194364Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq
Other Designations
ATP-dependent DNA helicase II|DNA repair protein XRCC5|Ku autoantigen, 80kDa|Ku86 autoantigen related protein 1|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kD)|X-ray repair, comp
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Interactome
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Pathway
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Disease
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