XRCC5 monoclonal antibody, clone 5C5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) against HeLa (1), MCF-7 (2), A-549 (3) and NIH/3T3 (4) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue (A) and human colon cancer tissue (B) using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HeLa cells using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant XRCC5.
Immunogen
Recombinant protein corresponding to human XRCC5.
Host
Mouse
Theoretical MW (kDa)
86
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) against HeLa (1), MCF-7 (2), A-549 (3) and NIH/3T3 (4) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue (A) and human colon cancer tissue (B) using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) (green) and negative control (purple). -
Gene Info — XRCC5
Entrez GeneID
7520Gene Name
XRCC5
Gene Alias
FLJ39089, KARP-1, KARP1, KU80, KUB2, Ku86, NFIV
Gene Description
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Omim ID
194364Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq
Other Designations
ATP-dependent DNA helicase II|DNA repair protein XRCC5|Ku autoantigen, 80kDa|Ku86 autoantigen related protein 1|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kD)|X-ray repair, comp
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Interactome
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Pathway
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Disease
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