Cycs monoclonal antibody, clone 6H2.B4
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More Files
- More Functions
Immunoprecipitation
Detection of Cycs in HeLa cells.
Two micrograms of 6H2.B4 (Lane 1) or control IgG (Lane 2) was used to immunoprecipitate Cycs from 1 x 106 cells and the immunoprecipitated protein was resolved on SDS-PAGE.
Cycs was detected by WB using Cycs monoclonal antibody, clone 6H2.B4 (Cat #MAB6240, 2 ug/mL).
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Specification
Product Description
Mouse monoclonal antibody raised against Cycs.
Immunogen
Rat Cycs.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
Clone 6H2.B4 (IMG-102A) recognizes the native form of Cytochrome c (Goshorn et al. 1991). Native cytochrome c has an approximate molecular weight of 15 KDa. Clone 6H2. B4 does not appear to recognize the denatured form of Cytochrome c. This antibody recognizes an epitope around residue 62 of rat Cytochrome c, based on copetitive ELISA results (Goshorn et al. 1991).
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Immunoprecipitation (2 ug/106 cells)
Immunohistochemistry (Frozen sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% gelatin, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Detection of Cycs in HeLa cells.
Two micrograms of 6H2.B4 (Lane 1) or control IgG (Lane 2) was used to immunoprecipitate Cycs from 1 x 106 cells and the immunoprecipitated protein was resolved on SDS-PAGE.
Cycs was detected by WB using Cycs monoclonal antibody, clone 6H2.B4 (Cat #MAB6240, 2 ug/mL). -
Gene Info — Cycs
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Publication Reference
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Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3.
Zou H, Henzel WJ, Liu X, Lutschg A, Wang X.
Cell 1997 Aug; 90(3):405.
Application：WB-Tr, Human, HEK 293 cells.
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Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c.
Liu X, Kim CN, Yang J, Jemmerson R, Wang X.
Cell 1996 Jul; 86(1):147.
Application：Immunodepletion, Human, HeLa S-100 cells.
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CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme.
Fernandes-Alnemri T, Litwack G, Alnemri ES.
The Journal of Biological Chemistry 1994 Dec; 269(49):30761.
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Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3.
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