Itgae monoclonal antibody, clone 2E7
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More Files
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Flow Cytometry
BALB/c mesenteric lymph node cells were first labeled with Itgae monoclonal antibody, clone 2E7 (Biotin), and then double-stained with streptavidin-FITC and Cd8a monoclonal antibody, clone 53-6.7 (PE) (Cat # MAB6107). Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Hamster monoclonal antibody raised against native Itgae.
Immunogen
Native purified from mouse intestinal intraepithelial lymphocytes.
Host
Hamster
Reactivity
Mouse
Specificity
The CD103/alphaIELbeta7 integrin complex (Mr 160 and 115 KDa peptides).
Form
Liquid
Isotype
IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c mesenteric lymph node cells were first labeled with Itgae monoclonal antibody, clone 2E7 (Biotin), and then double-stained with streptavidin-FITC and Cd8a monoclonal antibody, clone 53-6.7 (PE) (Cat # MAB6107). Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Itgae
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Publication Reference
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Co-expression of CD8 alpha in CD4+ T cell receptor alpha beta + T cells migrating into the murine small intestine epithelial layer.
Reimann J, Rudolphi A.
European Journal of Immunology 1995 Jun; 25(6):1508.
Application：Flow Cyt, Mouse, Mouse T cells.
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Developmental expression of the alpha IEL beta 7 integrin on T cell receptor gamma delta and T cell receptor alpha beta T cells.
Lefrancois L, Barrett TA, Havran WL, Puddington L.
European Journal of Immunology 1994 Mar; 24(3):635.
Application：Flow Cyt, Mouse, Thymocyte.
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The mucosal T cell integrin alpha M290 beta 7 recognizes a ligand on mucosal epithelial cell lines.
Roberts K, Kilshaw PJ.
European Journal of Immunology 1993 Jul; 23(7):1630.
Application：Cytotoxicity, Mouse, CMT93 cells.
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Co-expression of CD8 alpha in CD4+ T cell receptor alpha beta + T cells migrating into the murine small intestine epithelial layer.
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