Cd44 monoclonal antibody, clone KM201
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More Files
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Flow Cytometry
Cells from BALB/c bone marrow were stained with Cd44 monoclonal antibody, clone KM201 (FITC), following which large cells were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Cd44.
Immunogen
(C57 BL/6 x DBA/2)F1 mouse bone marrow derived stromal cells clone BM52.
Host
Rat
Reactivity
Mouse
Specificity
All isoforms of CD44/pgp-1 (Mr 80-95 KDa).
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Western Blot
Immunoprecipitation
Flow Cytometry
Cells from BALB/c bone marrow were stained with Cd44 monoclonal antibody, clone KM201 (FITC), following which large cells were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd44
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Publication Reference
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The CD44 expressed on the earliest intrathymic precursor population functions as a thymus homing molecule but does not bind to hyaluronate.
Wu L, Kincade PW, Shortman K.
Immunology Letters 1993 Sep; 38(1):69.
Application：Flow Cyt, Mouse, Thymocyte.
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Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.
Lesley J, He Q, Miyake K, Hamann A, Hyman R, Kincade PW.
The Journal of Experimental Medicine 1992 Jan; 175(1):257.
Application：Flow Cyt, Func, Mouse, AKR1, BW5147, SAKRTLS, TEM-3H3 cells.
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Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures.
Miyake K, Medina KL, Hayashi S, Ono S, Hamaoka T, Kincade PW.
The Journal of Experimental Medicine 1990 Feb; 171(2):477.
Application：Flow Cyt, IA, IP, Mouse, BM-2 hybridoma cells, Mouse bone marrow, Mouse bone marrow cells, Mouse lymph node, Mouse spleen, Mouse Thymus.
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The CD44 expressed on the earliest intrathymic precursor population functions as a thymus homing molecule but does not bind to hyaluronate.
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