SPN monoclonal antibody, clone TP1/36
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Specification
Product Description
Mouse monoclonal antibody raised against native SPN.
Immunogen
Native purified SPN from myeloblastic cell line KG1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 uL/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 1% BSA, pH 7.2 (0.09% sodium azide).
Storage Instruction
Store in the dark at 4°C. Avoid prolonged exposure to light.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
Flow Cytometry using human peripheral blood cells (1 ug/106 cells).
FACSCalibur (Becton Dickinson, San Jose, CA) flow cytometer, using Cell Quest acquisition software and PAINT-A-GATE. PRO, analysis software. -
Gene Info — SPN
Entrez GeneID
6693Gene Name
SPN
Gene Alias
CD43, GPL115, LSN
Gene Description
sialophorin
Omim ID
182160Gene Ontology
HyperlinkGene Summary
Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.[supplied by OMIM
Other Designations
leukosialin|sialophorin (gpL115, leukosialin, CD43)|sialophorin (leukosialin, CD43)
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Interactome
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Pathway
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Disease
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Publication Reference
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Immunophenotypic characterization of human bone marrow mast cells. A flow cytometric study of normal and pathological bone marrow samples.
Escribano L, Orfao A, Villarrubia J, Diaz-Agustin B, Cervero C, Rios A, Velasco JL, Ciudad J, Navarro JL, San Miguel JF.
Analytical Cellular Pathology 1998 Jan; 16(3):151.
Application:Flow Cyt, Human, Human bone marrow samples.
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An immunohistochemical study of mononuclear cells in meningiomas.
Bo L, Mork SJ, Nyland H.
Neuropathology and Applied Neurobiology 1992 Dec; 18(6):548.
Application:IHC-P, Human, Human meningiomas.
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CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes.
Borche L, Lozano F, Vilella R, Vives J.
European Journal of Immunology 1987 Oct; 17(10):1523.
Application:Flow Cyt, IP, Human, Leukocytes, Thymus cells.
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Immunophenotypic characterization of human bone marrow mast cells. A flow cytometric study of normal and pathological bone marrow samples.
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