VCP monoclonal antibody, clone Hs-14
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More Files
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Immunofluorescence
Immunofluorescence analysis of 220 kDa intra-acrosomal protein in acetone-permeabilized human sperms using VCP monoclonal antibody, clone Hs-14 (Cat # MAB3824) (normal spermiogram).
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Specification
Product Description
Mouse monoclonal antibody raised against native purified ACRV1.
Immunogen
Native purified human VCP.
Host
Mouse
Theoretical MW (kDa)
220
Reactivity
Human, Mouse
Specificity
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
Form
Liquid
Isotype
IgM
Recommend Usage
Immunocytochemistry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of 220 kDa intra-acrosomal protein in acetone-permeabilized human sperms using VCP monoclonal antibody, clone Hs-14 (Cat # MAB3824) (normal spermiogram).Flow Cytometry
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Gene Info — VCP
Entrez GeneID
7415Gene Name
VCP
Gene Alias
IBMPFD, MGC131997, MGC148092, MGC8560, TERA, p97
Gene Description
valosin-containing protein
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq
Other Designations
transitional endoplasmic reticulum ATPase|yeast Cdc48p homolog
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Interactome
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Disease
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Publication Reference
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[Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction].
Pavlásek J, Peknicová J, Ulcová-Gallová Z, Nováková P, Reischig J, Micanová Z, Rokyta Z.
Ceska Gynekol 2004 Jul; 69(4):306.
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Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage.
Peknicová J, Kyselová V, Buckiová D, Boubelík M.
American Journal of Reproductive Immunology 2002 May; 47(5):311.
Application：IF, WB-Ce, Dog, Human, Mouse, Sperm.
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[Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction].
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