S100A1 monoclonal antibody, clone SPM354
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human melanoma with S100A1 monoclonal antibody, clone SPM354 (Cat # MAB2546) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Mouse monoclonal antibody raised against native S100A1.
Immunogen
Native purified bovine brain S100A1.
Host
Mouse
Theoretical MW (kDa)
22, 11
Reactivity
Bovine, Human, Mouse, Rat
Specificity
This antibody recognizes 22 KDa for non-reduced S100A1 and 11 KDa for reduced S100A1.
Form
Liquid
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human melanoma with S100A1 monoclonal antibody, clone SPM354 (Cat # MAB2546) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — S100A1
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