Detyrosinated alpha Tubulin monoclonal antibody, clone RM444
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1:HeLa, Lane 2:U-2 OS, Lane 3:CHL-1 cell lysates with Detyrosinated alpha Tubulin monoclonal antibody, clone RM444 (Cat # MAB23229) at a 1:1000 dilution.
Immunocytochemistry
Immunocytochemical staining of CHL-1 using Detyrosinated alpha Tubulin monoclonal antibody, clone RM444 (Cat # MAB23229) at a 1:200 dilution (pink), anti-alpha-Tubulin (green) antibody, and DAPI (blue). Bar: 20 um.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against detyrosinated alpha-tubulin.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to detyrosinated alpha-tubulin.
Reactivity
Human
Specificity
This antibody reacts to Detyrosinated alpha Tubulin a PTM of α-tubulin where the C-terminal tyrosine residue has been cleaved.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:100-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1:HeLa, Lane 2:U-2 OS, Lane 3:CHL-1 cell lysates with Detyrosinated alpha Tubulin monoclonal antibody, clone RM444 (Cat # MAB23229) at a 1:1000 dilution.Immunocytochemistry
Immunocytochemical staining of CHL-1 using Detyrosinated alpha Tubulin monoclonal antibody, clone RM444 (Cat # MAB23229) at a 1:200 dilution (pink), anti-alpha-Tubulin (green) antibody, and DAPI (blue). Bar: 20 um.
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