Drd1a monoclonal antibody, clone SG2-D1a
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More Files
- More Functions
Western Blot (Transfected lysate)
Detection of Drd1a of Sf9 cells transfected with rat Drd1a using Drd1a monoclonal antibody, clone SG2-D1a (Cat # MAB2314).
Lane 1 : Sf9-Drd5 lysate.
Lane 2 : Sf9-Drd1a lyate.
Please note that these results are similiar to those shown in J. Neuroimmunol. 101 : 170-187 publication.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Drd1a.
Immunogen
Recombinant protein corresponding to C-terminus of rat Drd1a.
Host
Mouse
Reactivity
Mouse, Rat
Specificity
This antibody reacts with Dopamine Receptor D1A. There is no cross- reactivity with D1B (D5) receptor.
Form
Liquid
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Detection of Drd1a of Sf9 cells transfected with rat Drd1a using Drd1a monoclonal antibody, clone SG2-D1a (Cat # MAB2314).
Lane 1 : Sf9-Drd5 lysate.
Lane 2 : Sf9-Drd1a lyate.
Please note that these results are similiar to those shown in J. Neuroimmunol. 101 : 170-187 publication.Immunocytochemistry
Immunofluorescence
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Gene Info — Drd1a
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Publication Reference
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Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay.
Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T.
Scientific Reports 2015 Jun; 5:11333.
Application：IF, IP, WB, Mouse, Spleen cells were fused with SP2 myeloma cell line.
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Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes.
Luedtke RR, Griffin SA, Conroy SS, Jin X, Pinto A, Sesack SR.
Journal of Neuroimmunology 1999 Nov; 101(2):170.
Application：IHC, WB-Ti, WB-Tr, Insect, Rat, Sf9 cells, Brain.
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Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay.
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