Cytokeratin 5/6/18 monoclonal antibody, clone LP34
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Immunohistochemistry
Immunohistochemical staining of human skin: localized throughout the epidermis, with the strongest staining in the stratum spinosum. There is an absence of staining in the dermis.
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Specification
Product Description
Mouse monoclonal antibody raised against human cytokeratin 5, 6 and 18.
Immunogen
Detergent-insoluble fraction of psoriatic human epidermis.
Host
Mouse
Reactivity
Human
Specificity
Human cytokeratin 5, 6 and 18 intermediate filament proteins.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunohistochemistry (On paraffin sections. Enzyme Induced Epitope Retrieval (EIER)) (1:100 for 30 minutes at 25°C)
The optimal working dilution should be determined by the end user.
This antibody reacts with human cytokeratin intermediate filament proteins 5, 6 and 18 on frozen tissue. The staining of cytokeratin 18 may be variable on some formalin-fixed, paraffin-embedded tissue sections with this antibody.Storage Buffer
In tissue culture supernatant (0.09% sodium azide).
Storage Instruction
Store at 4°C. Do not freeze. Return to 2-8°C immediately after use. Do not use after expiration date indicated on the vial label.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Recommended positive control for immunohistochemistry is skin. -
Applications
Immunohistochemistry
Immunohistochemical staining of human skin: localized throughout the epidermis, with the strongest staining in the stratum spinosum. There is an absence of staining in the dermis.
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