Mouse Anti-Human IgM secondary antibody, clone UHB (Biotin)
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Specification
Product Description
Mouse monoclonal antibody raised against human IgM.
Immunogen
Purified human IgM.
Host
Mouse
Theoretical MW (kDa)
900
Reactivity
Human
Specificity
The antibody reacts with human IgM.
Form
Liquid
Conjugation
Biotin
Purification
Precipitation and/or chromatography
Purity
>= 90% purity by HPLC and/or SDS-PAGE
Isotype
IgG3, kappa
Recommend Usage
ELISA (1:5000-1:10000)
Flow Cytometry (<= 1 ug/106cells): suggested use of the reagent is in a final volume of 100 uL
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgM secondary antibody, clone UHB (Biotin).Flow Cytometry
Flow cytometric analysis of human peripheral blood lymphocytes were stained with Mouse Anti-Human IgM secondary antibody, clone UHB (Biotin) (Cat # MAB22719) and Mouse Anti-Human CD19 secondary antibody (PE) followed by Streptavidin-FITC. -
Gene Info — IGHM
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Interactome
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Disease
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