YWHAQ monoclonal antibody, clone AT1A1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MDA-MB-21 cell lysate, Lane 2: HeLa cell lysate, Lane 3: A431 cell lysate, Lane 4: NIH3T3 cell lysate, Lane 5: 293T cell lysate, Lane 6: HepG2 cell lysate.
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Recombinant Human YWHAZ, Lane 2: Recombinant Human YWHAB, Lane 3: Recombinant Human YWHAE, Lane 4: Recombinant Human YWHAH, Lane 5: Recombinant Human YWHAG, Lane 6: Recombinant Human SFN, Lane 7: Recombinant Human YWHAQ.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with YWHAQ monoclonal antibody, clone AT1A1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant YWHAQ.
Immunogen
Recombinant protein corresponding to amino acids 1-245 of human YWHAQ.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MDA-MB-21 cell lysate, Lane 2: HeLa cell lysate, Lane 3: A431 cell lysate, Lane 4: NIH3T3 cell lysate, Lane 5: 293T cell lysate, Lane 6: HepG2 cell lysate.Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Recombinant Human YWHAZ, Lane 2: Recombinant Human YWHAB, Lane 3: Recombinant Human YWHAE, Lane 4: Recombinant Human YWHAH, Lane 5: Recombinant Human YWHAG, Lane 6: Recombinant Human SFN, Lane 7: Recombinant Human YWHAQ.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with YWHAQ monoclonal antibody, clone AT1A1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — YWHAQ
Entrez GeneID
10971Protein Accession#
NP_006817Gene Name
YWHAQ
Gene Alias
14-3-3, 1C5, HS1
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Omim ID
609009Gene Ontology
HyperlinkGene Summary
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq
Other Designations
14-3-3 protein T-cell|14-3-3 protein tau|14-3-3 protein theta|OTTHUMP00000015730|protein tau|tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide
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Interactome
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Pathway
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Disease
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Publication Reference
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Activation-modulated association of 14-3-3 proteins with Cbl in T cells.
Liu YC, Elly C, Yoshida H, Bonnefoy-Berard N, Altman A.
The Journal of Biological Chemistry 1996 Jun; 271(24):14591.
Application：WB-Ce, Human, Jurkat cells.
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Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways.
Xiao B, Smerdon SJ, Jones DH, Dodson GG, Soneji Y, Aitken A, Gamblin SJ.
Nature 1995 Jul; 376(6536):188.
Application：WB-Ce, WB-Tr, Human, Mammalian cells.
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Activation-modulated association of 14-3-3 proteins with Cbl in T cells.
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