WNT3A monoclonal antibody, clone 3A6
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: Wnt3a Transfected 293T cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast cancer tissue were incubated with WNT3A monoclonal antibody, clone 3A6 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant WNT3A.
Immunogen
Recombinant protein corresponding to amino acids 19-352 of human WNT3A.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: Wnt3a Transfected 293T cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast cancer tissue were incubated with WNT3A monoclonal antibody, clone 3A6 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
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Gene Info — WNT3A
Entrez GeneID
89780Protein Accession#
NP_149122Gene Name
WNT3A
Gene Alias
MGC119418, MGC119419, MGC119420
Gene Description
wingless-type MMTV integration site family, member 3A
Omim ID
606359Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq
Other Designations
OTTHUMP00000035713
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Interactome
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Pathway
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Disease
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Publication Reference
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Human umbilical cord mesenchymal stem cells implantation accelerates cutaneous wound healing in diabetic rats via the Wnt signaling pathway.
Han Y, Sun T, Han Y, Lin L, Liu C, Liu J, Yan G, Tao R.
European Journal of Medical Research 2019 Feb; 24(1):10.
Application：Cell Cuture, Human, hUCMSCs.
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Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation.
Pan W, Choi SC, Wang H, Qin Y, Volpicelli-Daley L, Swan L, Lucast L, Khoo C, Zhang X, Li L, Abrams CS, Sokol SY, Wu D.
Science 2008 Sep; 321(5894):1350.
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Regulation of WNT3 and WNT3A mRNAs in human cancer cell lines NT2, MCF-7, and MKN45.
Katoh M.
International Journal of Oncology 2002 Feb; 20(2):373.
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Wnt family proteins are secreted and associated with the cell surface.
Smolich BD, McMahon JA, McMahon AP, Papkoff J.
Molecular Biology of the Cell. 1993 Dec; 4(12):1267.
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Human umbilical cord mesenchymal stem cells implantation accelerates cutaneous wound healing in diabetic rats via the Wnt signaling pathway.
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