YAP1 (phospho S127) monoclonal antibody, clone GAB-25
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) HeLa cell lysate, (2) HeLa cell lysate treated with FBS+calyculin A using YAP1 (phospho S127) monoclonal antibody, clone GAB-25.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human YAP1.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S127 of human YAP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) HeLa cell lysate, (2) HeLa cell lysate treated with FBS+calyculin A using YAP1 (phospho S127) monoclonal antibody, clone GAB-25.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — YAP1
Entrez GeneID
10413Protein Accession#
P46937Gene Name
YAP1
Gene Alias
YAP, YAP2, YAP65, YKI
Gene Description
Yes-associated protein 1, 65kDa
Omim ID
606608Gene Ontology
HyperlinkGene Summary
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq
Other Designations
yes-associated protein 2
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Interactome
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Disease
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