DUSP1 monoclonal antibody, clone IHB-4
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: NIH/3T3 and Lane 3: PC-12 cell lysates with DUSP1 monoclonal antibody, clone IHB-4 (Cat # MAB19992).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human DUSP1.
Immunogen
A synthetic peptide corresponding to human DUSP1.
Host
Rabbit
Theoretical MW (kDa)
39.298
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: NIH/3T3 and Lane 3: PC-12 cell lysates with DUSP1 monoclonal antibody, clone IHB-4 (Cat # MAB19992).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — DUSP1
Entrez GeneID
1843Protein Accession#
P28562Gene Name
DUSP1
Gene Alias
CL100, HVH1, MKP-1, MKP1, PTPN10
Gene Description
dual specificity phosphatase 1
Omim ID
600714Gene Ontology
HyperlinkGene Summary
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq
Other Designations
serine/threonine specific protein phosphatase
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Interactome
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Pathway
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Disease
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