P2RY1 monoclonal antibody, clone 4G5B7
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human P2RY1.
Immunogen
Recombinant protein corresponding to amino acid of human P2RY1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
42
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) P2RY1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with P2RY1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of P2RY1 monoclonal antibody, clone 4G5B7.Flow Cytometry
Flow cytometric analysis of Hela cells with P2RY1 monoclonal antibody (green) and negative control (red). -
Gene Info — P2RY1
Entrez GeneID
5028Gene Name
P2RY1
Gene Alias
P2Y1
Gene Description
purinergic receptor P2Y, G-protein coupled, 1
Omim ID
601167Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq
Other Designations
ATP receptor|P2 purinoceptor subtype Y1|P2Y purinoceptor 1|platelet ADP receptor|purinergic receptor P2Y1
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Interactome
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