MAP2K3 monoclonal antibody, clone 2E12D11
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) HEK293 cells, (2) MAP2K3-hIgGFc transfected HEK293 cell lysate.
Western Blot
Western Blot analysis of human MAP2K3 recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) bladder cancer tissues and (B) rectum cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of (A) GC-7901 (B) HeLa and (C) HepG2 cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MAP2K3 monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP2K3 mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human MAP2K3.
Immunogen
Recombinant protein corresponding to amino acids 1-138 of of human MAP2K3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
39.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HEK293 cells, (2) MAP2K3-hIgGFc transfected HEK293 cell lysate.Western Blot
Western Blot analysis of human MAP2K3 recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) bladder cancer tissues and (B) rectum cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of (A) GC-7901 (B) HeLa and (C) HepG2 cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MAP2K3 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP2K3 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — MAP2K3
Entrez GeneID
5606Gene Name
MAP2K3
Gene Alias
MAPKK3, MEK3, MKK3, PRKMK3
Gene Description
mitogen-activated protein kinase kinase 3
Omim ID
602315Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq
Other Designations
MAP kinase kinase 3|MAPK/ERK kinase 3|OTTHUMP00000166044|dual specificity mitogen activated protein kinase kinase 3
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Interactome
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Pathway
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