UFD1L monoclonal antibody, clone 4F11A4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) UFD1L-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with UFD1L monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with UFD1L monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of UFD1L monoclonal antibody, clone 4F11A4.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human UFD1L.
Immunogen
Recombinant protein corresponding to amino acid 208-307 of human UFD1L from E. coli.
Host
Mouse
Theoretical MW (kDa)
34.5kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) UFD1L-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with UFD1L monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with UFD1L monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of UFD1L monoclonal antibody, clone 4F11A4. -
Gene Info — UFD1L
Entrez GeneID
7353Gene Name
UFD1L
Gene Alias
UFD1
Gene Description
ubiquitin fusion degradation 1 like (yeast)
Omim ID
601754Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. [provided by RefSeq
Other Designations
UB fusion protein 1|ubiquitin fusion degradation 1-like|ubiquitin fusion degradation protein 1 homolog
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Interactome
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Disease
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