SEC31A monoclonal antibody, clone 5D12C6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) SEC31A-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with SEC31A monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SEC31A monoclonal antibody, clone 5D12C6.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SEC31A.
Immunogen
Recombinant protein corresponding to amino acid 429-571 of human SEC31A from E. coli.
Host
Mouse
Theoretical MW (kDa)
133kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) SEC31A-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with SEC31A monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SEC31A monoclonal antibody, clone 5D12C6. -
Gene Info — SEC31A
Entrez GeneID
22872Gene Name
SEC31A
Gene Alias
ABP125, ABP130, DKFZp686N07171, HSPC275, HSPC334, KIAA0905, MGC90305, SEC31L1
Gene Description
SEC31 homolog A (S. cerevisiae)
Omim ID
610257Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII , in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000160810|SEC31 homolog A|SEC31-like 1|protein-transport protein SEC31|yeast Sec31p homolog
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Interactome
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Disease
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