GFPT1 monoclonal antibody, clone 1F1B9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: HepG2 cell; Lane 3: HEK293 cell; Lane 4: BEL-7402 cell; Lane 5: SMMC-7721 cell; Lane 6: SK-MES-1 cell; Lane 7: C6 cell; Lane 8: COS-7 cell with GFPT1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) GFPT1-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with GFPT1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GFPT1 monoclonal antibody, clone 1F1B9.
Flow Cytometry
Flow cytometric analysis of HeLa cells with GFPT1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human GFPT1.
Immunogen
Recombinant protein corresponding to amino acid 536-681 of human GFPT1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
78.8kDa
Reactivity
Human, Monkey, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: HepG2 cell; Lane 3: HEK293 cell; Lane 4: BEL-7402 cell; Lane 5: SMMC-7721 cell; Lane 6: SK-MES-1 cell; Lane 7: C6 cell; Lane 8: COS-7 cell with GFPT1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) GFPT1-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with GFPT1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GFPT1 monoclonal antibody, clone 1F1B9.Flow Cytometry
Flow cytometric analysis of HeLa cells with GFPT1 monoclonal antibody (green) and negative control (red). -
Gene Info — GFPT1
Entrez GeneID
2673Gene Name
GFPT1
Gene Alias
GFA, GFAT, GFAT1, GFAT1m, GFPT
Gene Description
glutamine-fructose-6-phosphate transaminase 1
Omim ID
138292Gene Ontology
HyperlinkGene Summary
This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq
Other Designations
glucosamine-fructose-6-phosphate aminotransferase|glutamine: fructose-6-phosphate amidotransferase-1
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Interactome
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Pathway
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Disease
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