RND3 monoclonal antibody, clone 5C7B6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RND3-hIgGFc transfected HEK293 cell with RND3 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with RND3 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with RND3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RND3 monoclonal antibody, clone 5C7B6.
Flow Cytometry
Flow cytometric analysis of Hela cells with RND3 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RND3.
Immunogen
Recombinant protein corresponding to amino acid 104-241 of human RND3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
27.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RND3-hIgGFc transfected HEK293 cell with RND3 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with RND3 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with RND3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RND3 monoclonal antibody, clone 5C7B6.Flow Cytometry
Flow cytometric analysis of Hela cells with RND3 monoclonal antibody (green) and negative control (red). -
Gene Info — RND3
Entrez GeneID
390Gene Name
RND3
Gene Alias
ARHE, Rho8, RhoE, memB
Gene Description
Rho family GTPase 3
Omim ID
602924Gene Ontology
HyperlinkGene Summary
Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).[supplied by OMIM
Other Designations
OTTHUMP00000162685|ras homolog gene family, member E|small GTP binding protein Rho8
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