RALA monoclonal antibody, clone 4G8C7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: MCF-7 cell; Lane 3: A549 cell; Lane 4: K562 cell; Lane 5: Raji cell; Lane 6: MOLT4 cell; Lane 7: Hela cell; Lane 8: COS7 cell and Lane 9: NIH/3T3 cell with RALA monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RALA-hIgGFc transfected HEK293 cell with RALA monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with RALA monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with RALA monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RALA monoclonal antibody, clone 4G8C7.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with RALA monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RALA.
Immunogen
Recombinant protein corresponding to amino acid 71-203 of human RALA from E. coli.
Host
Mouse
Theoretical MW (kDa)
23.6
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: MCF-7 cell; Lane 3: A549 cell; Lane 4: K562 cell; Lane 5: Raji cell; Lane 6: MOLT4 cell; Lane 7: Hela cell; Lane 8: COS7 cell and Lane 9: NIH/3T3 cell with RALA monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RALA-hIgGFc transfected HEK293 cell with RALA monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with RALA monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with RALA monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RALA monoclonal antibody, clone 4G8C7.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with RALA monoclonal antibody (green) and negative control (red). -
Gene Info — RALA
Entrez GeneID
5898Gene Name
RALA
Gene Alias
MGC48949, RAL
Gene Description
v-ral simian leukemia viral oncogene homolog A (ras related)
Omim ID
179550Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq
Other Designations
RAS-like protein A|Ras family small GTP binding protein RALA|Ras-related protein Ral-A|ras related v-ral simian leukemia viral oncogene homolog A
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Interactome
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Pathway
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Disease
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