PDPK1 monoclonal antibody, clone 7E4G11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: Hela cell; Lane 3: K562 cell; Lane 4: U937 cell; Lane 5: A549 cell; Lane 6: NIH/3T3 cell; Lane 7: Jurkat cell; Lane 8: PC-12 cell and Lane 9: Cos7 cell with PDPK1 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PDPK1-hIgGFc transfected HEK293 cell with PDPK1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with PDPK1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of A549 cells with PDPK1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PDPK1 monoclonal antibody, clone 7E4G11.
Flow Cytometry
Flow cytometric analysis of A549 cells with PDPK1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PDPK1.
Immunogen
Recombinant protein corresponding to amino acid 457-556 of human PDPK1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
63.2
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:50-1:200)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: Hela cell; Lane 3: K562 cell; Lane 4: U937 cell; Lane 5: A549 cell; Lane 6: NIH/3T3 cell; Lane 7: Jurkat cell; Lane 8: PC-12 cell and Lane 9: Cos7 cell with PDPK1 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PDPK1-hIgGFc transfected HEK293 cell with PDPK1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with PDPK1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of A549 cells with PDPK1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PDPK1 monoclonal antibody, clone 7E4G11.Flow Cytometry
Flow cytometric analysis of A549 cells with PDPK1 monoclonal antibody (green) and negative control (red). -
Gene Info — PDPK1
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Interactome
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Pathway
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Disease
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