Polyethylene glycol (methoxy group) monoclonal antibody, clone RM105 (Biotin)
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More Files
- More Functions
Western Blot
Western blot analysis of BSA and PEGylated BSA with Polyethylene glycol (methoxy group) monoclonal antibody, clone RM105 (Biotin) (Cat # MAB14965) at 0.1 ug/mL working concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of mouse liver (mouse had been injected with PEG-BSA for 3 hours) (A), mouse liver (mouse had been injected with BSA for 3 hours) (B), mouse kidney (mouse had been injected with PEG-BSA for 3 hours) (C), and mouse kidney (mouse had been injected with BSA for 3 hours) (D) using Polyethylene glycol (methoxy group) monoclonal antibody, clone RM105 (Biotin) (Cat # MAB14965).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of different PEGylated BSA with Polyethylene glycol (methoxy group) monoclonal antibody, clone RM105 (Biotin) (Cat # MAB14965) at the following concentrations: 1 ug/mL, 0.33 ug/mL, 0.11 ug/mL, 0.04 ug/mL. The plate was coated with 50 uL/well different amounts of BSA-PEG. An alkaline phosphatase conjugated streptavidin as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against Polyethylene glycol (methoxy group).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against polyethylene glycol (methoxy group) conjugated with KLH.
Sequence
N/A
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.02-0.5 ug/mL)
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
Immunoprecipitation
Western Blotting (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of BSA and PEGylated BSA with Polyethylene glycol (methoxy group) monoclonal antibody, clone RM105 (Biotin) (Cat # MAB14965) at 0.1 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of mouse liver (mouse had been injected with PEG-BSA for 3 hours) (A), mouse liver (mouse had been injected with BSA for 3 hours) (B), mouse kidney (mouse had been injected with PEG-BSA for 3 hours) (C), and mouse kidney (mouse had been injected with BSA for 3 hours) (D) using Polyethylene glycol (methoxy group) monoclonal antibody, clone RM105 (Biotin) (Cat # MAB14965).Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of different PEGylated BSA with Polyethylene glycol (methoxy group) monoclonal antibody, clone RM105 (Biotin) (Cat # MAB14965) at the following concentrations: 1 ug/mL, 0.33 ug/mL, 0.11 ug/mL, 0.04 ug/mL. The plate was coated with 50 uL/well different amounts of BSA-PEG. An alkaline phosphatase conjugated streptavidin as the secondary antibody.Flow Cytometry
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